Spectroscopy: Theory and Application

Curriculum Guideline

Effective Date:
Course
Discontinued
No
Course Code
CHEM 2360
Descriptive
Spectroscopy: Theory and Application
Department
Chemistry
Faculty
Science & Technology
Credits
5.00
Start Date
End Term
201730
PLAR
No
Semester Length
15
Max Class Size
18
Contact Hours
Lecture 4 hours Laboratory 3 hours
Method(s) Of Instruction
Lecture
Lab
Learning Activities

The course will be presented using lectures, classroom demonstrations, problem sessions, on-line quizzes, and class discussions. Audio-visual materials, including molecular modeling software, will be used where appropriate. The laboratory course will be used to illustrate the practical aspects of the course material, using both traditional wet labs and computer techniques. Close coordination will be maintained between laboratory and classroom work whenever possible. 

Course Description
This course introduces the principles of quantum mechanics as they apply to atomic and molecular spectroscopy. The following techniques are then covered from both a theoretical and practical perspective: Infrared Spectroscopy, Raman Spectroscopy, UV-VIS Spectroscopy, NMR Spectroscopy, Atomic Spectroscopy and GC-Mass Spectrometry. The experimental application of this material will be covered in both wet-bench and computational laboratory techniques.
Course Content

Introduction and Principles of Quantum Mechanics

The electromagnetic spectrum. Wavefunctions. The Schrödinger Equation. Particle in a Box.  Atomic and Molecular Energy Levels. Electronic Transitions. Boltzmann Distribution.

Symmetry and Spectroscopy

Symmetry operators and point groups and their relation to spectroscopy.

Atomic Structure and Spectroscopy

H atom and multi-electron atoms. Atomic Orbitals. Term Symbols. Atomic Spectra.

Molecular Rotations

Rotational motion. Selection Rules. Raman Spectroscopy.

Molecular Vibrations

Vibrational motion. Selection Rules. Energy Levels. Infrared Spectroscopy.    

Magnetic Resonance Spectroscopy

Nuclear spin. Splitting of energy levels in an applied magnetic field.  Molecular structure and chemical shifts.  NMR Spectroscopy of proton and multi-nuclear species.

Mass Spectrometry

Magnetic sector, quadrupole and ion-trap techniques. High resolution spectral interpretation. Fragmentation patterns.

UV-Visible Spectroscopy

Energy levels and transitions in organic and coordination compounds. Spectra of pure compounds and mixtures.                       

Laboratory Content:

Experiments will be selected from the following list:

  1. Quantitative UV/Vis Spectroscopy of a Mixture
  2. Determination of Keto-Enol Equilibrium Constants by NMR
  3. Geometric Isomers of a Cr(III) Complex
  4. Preparation and Identification of Co(III) Complexes
  5. Paramagnetic Susceptibility by  NMR
  6. Computer Lab I: Use of EXCEL
  7. Computer Lab II: Molecular Modeling
  8. Computer Lab III: Spectral Simulation and Interpretation
  9. Infrared Spectroscopy of Liquid Samples
  10. Solid Sample Preparation Methods for Infrared Spectroscopy
  11. GC-MS Analysis of a Volatile Mixture
  12. Structural Determination by NMR: Esterification Reactions of Vanillin
  13. Term Project: Determination of an Unknown by IR, NMR, and GC-MS
  14. Atomic Absorption: Quantitative Analysis of a Metal
Learning Outcomes
  1. Understand the properties of the electromagnetic spectrum and how to calculate energy, wavelength, and frequency of light
  2. Describe the photoelectric effect and blackbody radiation
  3. Understand wave particle duality and the Heisenberg Uncertainty Principle
  4. Apply the Schrödinger Equation to Wavefunctions in order to understand how electronic energy levels are determined
  5. Apply the concepts of moment of inertia and particle in a box to determine allowed rotational excitations in a molecule
  6. Understand spin and the allowed transitions of electron and nuclear spin in an applied magnetic field
  7. Sketch the shapes of atomic orbitals from a set of quantum numbers
  8. Understand the Aufbau Principle, Pauli Exclusion Principle and the use of  Term Symbols for multi-electron atoms
  9. Understand bond formation in a diatomic molecule through the use of the Born-Oppenheimer Approximation
  10. Apply the Simple Harmonic Oscillator model to understand vibrations in diatomic molecules and simple polyatomics
  11. Apply selection rules to molecules to determine allowed vibrational modes
  12. Sketch simple vibrational modes in linear and non-linear polyatomic molecules
  13. Use the Boltzmann Distribution Law to describe energy levels in a molecule and predict the effect on spectroscopic results
  14. Given an infrared spectrum, determine the functional groups or coordinate bonds present using correlation charts
  15. Given an NMR spectrum, determine the number and type of protons present and the J-J coupling constants for first and second order
  16. Given the structure of a compound, predict the number of NMR peaks, chemical shifts, and splitting patterns using electronegativity, symmetry, and hybridization
  17. Interpret multi-nuclear NMR spectra of molecules containing 19F, 13C, and 31P by using chemical shifts, reference compounds, and decoupled spectra
  18. Given a UV-VIS spectrum, label the transitions occurring based on Molecular Orbital or Crystal Field splitting
  19. Calculate concentrations  or molar extinction coefficients by applying the Beer - Lambert Law to a UV-VIS spectrum
  20.  Analyze the UV-VIS spectrum of a two component mixture to determine individual concentrations
  21. Use information from given spectra (IR, UV-VIS, NMR, GC-MS) to determine the structural formula of  a compound
  22. Use EXCEL spreadsheets to create linear calibration curves, including error analysis
  23. Given a mass spectrum, predict the parent compound using fragmentation patterns
  24. Determine isotopic information from a high resolution mass spectrum
  25. For each spectroscopic method studied, be able to sketch the basic components of the instrument and understand their operation
  26. Prepare standards and unknown solutions as required for laboratory analysis and be able to run these samples and use instrumental software to analyze the spectra               
Means of Assessment

Lecture Material 70%

  • Two or three in-class tests will be given during the semester (30%)
  • A final exam covering the entire semester’s work will be given during the final examination period (30%)
  • Any or all of the following: problem assignments, on-line quizzes, class participation (10%)

Laboratory 30%

  • Each experiment and will be evaluated based on results and a written report. A formal report based on evaluation of an unknown will also be submitted.
Textbook Materials

Textbooks and Materials to be Purchased by Students

Required:

  • Pavia, Lampman, Kriz, and Vyvyan, Introduction to Spectrosopy, Current Edition, Brooks/Cole
  • Duckett and Gilbert, Foundations of Spectroscopy, Current Edition, Oxford University Press
  • ÁñÁ«ÊÓƵ, Chemistry 2360 Laboratory Manual.
  • Laboratory Notebook, Safety Goggles

Recommended:

  • Thomas Engel, Quantum Chemistry and Spectroscopy, 3rd Edition, Pearson, 2013
Prerequisites

CHEM 1110 (C or better)

MATH 1120 (Recommended)